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    Vector Laboratories vector labs pk
    Vector Labs Pk, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectastain+elite+abc+hrp+kit+vector+labs/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/us12473355-615-4-4
    Average 99 stars, based on 12897 article reviews
    vector labs pk - by Bioz Stars, 2026-10
    99/100 stars

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    Staining:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Purification:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Immunopeptidomics:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Transgenic Assay:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Marker:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: We thank Genmab for providing us with the CD3xTA99 bispecific antibodies. .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium hydrogen carbonate Merck Cat#: 106329 Sulfuric acid 95–97% Merck Cat#: 100731 TNF-alpha/TNFA/TNFSF2 Neutralizing Antibody SinoBiological Cat#: 50349-RN023 DETA NONOate (Noc18) MedChemExpress Cat#: HY-136278 Critical commercial assays Zombie UV fixable viability kit Biolegend Cat#: 423108 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit ThermoFisher Cat#: L34957 FoxP3/Transcription Factor Staining Buffer Set Invitrogen Cat#: 00-5523-00 VECTASTAIN Elite ABC-HRP Kit Vector Labs Cat#: PK-6100; RRID: AB_2336819 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#: C34554 Griess Reagent Kit ThermoFisher Cat#: G7921 Mouse CD8 T Lymphocyte Enrichment Set BD Biosciences Cat#: 558471 Dynabeads flowcomp CD8 kit Thermo Fisher Scientific Cat#: 11462D Mouse CCL3/MIP-1 alpha DuoSet ELISA R&D Systems Cat#: DY450 Mouse CCL4/MIP-1 beta DuoSet ELISA R&D Systems Cat#: DY451 Mouse CCL5/RANTES DuoSet ELISA R&D Systems Cat#: DY478 nCounter PanCancer Immune Profiling cartridge Nanostring Cat#: 115000142 nCounter Myeloid Innate Immunity Panel V2 Nanostring Cat#: XT-CSO-MMII2-12 NucleoSpin RNA mini kit for RNA purification Macherey-Nagel Cat#: 740955.50 High-Capacity RNA-to-cDNA Kit ThermoFisher Cat#: 4387406 Deposited data Raw and processed scRNA-seq data from MHC-II+ and MHC-II- intratumoral (RMA-Qa1 / ) murine macrophages This manuscript GEO: GSE263235 Raw and processed scRNA-seq data from intratumoral (CT26) murine macrophages Qu, Y. et al.42 GEO: GSE150970 Raw and processed scRNA-seq data from human monocyte/macrophage populations across tissues Mulder, K. et al.43 GEO: GSE178209 Raw and processed scRNA-seq data from human monocyte/macrophage and T cell populations in breast cancer Bassez, A. et al.48 EGA: EGAS00001004809 Experimental models: Cell lines RMA Dr. K. K€arre RRID: CVCL_J385 B16F10 ATCC Cat#: CRL6475 RRID: CVCL_0159 KPC3 Dr. F. Balkwill RRID: CVCL_A9ZK Experimental models: Organisms/strains Mouse C57BL/6J Charles River Laboratories France RRID: IMSR_JAX:000664 Mouse TCR transgenic gp10025-33/H-2D b bred to express congenic marker CD45.1 (Ly5.1) Provided by Dr. N.P. .. Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    other:

    Article Title: Immunotherapy-activated T cells recruit and skew late-stage activated M1-like macrophages that are critical for therapeutic efficacy.
    Article Snippet: Restifo (NIH, Bethesda, USA) N/A B6.129P2-Nos2tm1Lau/J (iNOS / ) The Jackson Laboratory RRID: IMSR_JAX:002609 Oligonucleotides See Table S3.

    Electron Microscopy:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated donkey anti-rabbit Jackson ImmunoResearch Labs Cat No: 711-065-152; RRID: AB_2340593 Biotinylated goat anti-mouse Vector Laboratories Cat No: BA-9200; RRID: AB_2336171 Chicken anti-Green Fluorescent Protein Aves Labs, Inc Cat No: GFP-1020; RRID: AB_10000240 Donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25810 Donkey anti-rabbit IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25701 Donkey anti-chicken Cy3 Jackson ImmunoResearch Labs Cat No: 703-165-155; RRID: AB_2340363 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Labs Cat No: 711-165-152; RRID: AB_2307443 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Labs Cat No: 711-225-152; RRID: AB_2340612 Donkey anti-sheep Cy5 Jackson ImmunoResearch Labs Cat No: 713-175-147; RRID: AB_2340730 Mouse anti-Tyrosine hydroxylase monoclonal Boehringer Mannheim Cat No: 1017-381 Rabbit anti-GABAAR a3 (extracellular) polyclonal Alomone Labs Cat No: AGA-003; RRID: AB_2039866 Rabbit anti-GABAAR a3 blocking peptide Alomone Labs Cat No: BLP-GA-003 Rabbit ant-GABAAR d (extracellular) Dr. W. Sieghart N/A Rabbit anti-Tyrosine hydroxylase polyclonal Millipore Cat No: AB152; RRID: AB_390204 Sheep anti-Tyrosine hydroxylase polyclonal Abcam Cat No: ab113; RRID: AB_297905 Bacterial and virus strains AAV8-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA pAAV-EF-1a-double floxedhChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth Addgene viral prep Cat No: 20297-AAV8 Chemicals, peptides, and recombinant proteins D-AP5 Hello Bio Cat No: HB0225 (+)Bicuculline Sigma-Aldrich Cat No: 14340 Calcium chloride dihydrate (CaCl2.2H2O) Sigma-Aldrich Cat No: C5080 DAB (3,30-Diaminobenzidine tetrahydrochloride) Sigma-Aldrich Cat No: D5905 DHbE hydrobromide Tocris Bioscience Cat No: 2349 DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat No: 276855 DNQX Hello Bio Cat No: HB0261 DOPAC (3,4-Dihydroxyphenylacetic acid) Sigma-Aldrich Cat No: D-9128 Dopamine hydrochloride (3- hydroxytryramine) Sigma-Aldrich Cat No: H-8502 EDTA (Ethylenediaminetetraacetic acid disodium salt dihydrate) Sigma-Aldrich Cat No: E1644 Ethanol, 200 proof Fisher Scientific Cat No: A962P-4 Euthasol Covetrus SKU No: 009444 Gabazine (SR 95531) Hello Bio Cat No: HB0901 D-(+)-Glucose Sigma-Aldrich Cat No: G-5767 HEPES (Acid) Sigma-Aldrich Cat No: H-3375 HEPES (Sodium Salt) Sigma-Aldrich Cat No: H-7006 Isoflurane Covetrus (NYULH-DCM) SKU No: 029405 (Continued on next page) 16 Cell Reports 43, 113834, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER JNJ-16259685 Tocris Bioscience Cat No: 2333 3-mercaptopropionic acid (3-MPA) Sigma-Aldrich Cat No: M5801 Magnesium sulfate heptahydrate (MgSO4.7H2O) Sigma-Aldrich Cat No: M-1880 Methanol Sigma-Aldrich Cat No: 34860 Muscimol Tocris Bioscience Cat No: 0289 Muscimol Hello Bio Cat No: HB0887 Normal Donkey Serum (NDS) Sigma-Aldrich Cat No: S30-M Paraformaldehyde Fisher Cat No: T353 Paraformaldehyde for EM (4% in 0.1M Phosphate Buffer, PH 7.4 Electron Microscopy Sciences Cat No: 15735-20S Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat No: P7059 Picrotoxin Sigma-Aldrich Cat No: P1675 Picrotoxin Hello Bio Cat No: HB0506 Potassium chloride (KCl) Sigma-Aldrich Cat No: P-3911 Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat No: P-0662 Sodium bicarbonate (NaHC03) Sigma-Aldrich Cat No: S-6014 Sodium-1-heptanesulfanate (C7H15O3SNa) Sigma-Aldrich Cat No: H2766 Sodium chloride (NaCl) Sigma-Aldrich Cat No: S-7653 Sodium octyl sulfate Sigma-Aldrich Cat No: O-4003 Sodium phosphate monobasic monohydrate (NaH2PO4.H2O) Sigma-Aldrich Cat No: S9638 Sucrose Sigma-Aldrich Cat No: S-7903 Strychnine hydrochloride Sigma-Aldrich Cat No: S8759 Triton X-100 Sigma-Aldrich Cat No: T8787 Critical commercial assays Silver Enhancer Kit for Microscopy Kirkegaard & Perry Laboratories (KPL), Inc Cat No: 50-22-01 Vectastain Elite ABC-HRP Kit Vector Labs Cat No: PK6100 Experimental models: Organisms/strains Mouse: Ai32 Jackson Laboratory Stock No: 024109 Mouse: C57BL/6J Jackson Laboratory Stock No: 000664 Mouse: DAT-IRES-Cre Jackson Laboratory Stock No: 006660 Mouse: GAT1flox/flox Tritsch Lab, NYULH geneOway JAX stock No: 037219 Software and algorithms DropViz RNA-Seq Data Saunders et al.48 https://DropViz.org Fiji ImageJ NIH https://imagej.nih.gov/ij/ Fusion Benchtop Image Acquisition Software (ver. .. 1.101) Andor Technology Ltd. Oxford Instruments https://andor.oxinst.com/ Imaris Microscope Imaging Software (ver.

    Saline:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated donkey anti-rabbit Jackson ImmunoResearch Labs Cat No: 711-065-152; RRID: AB_2340593 Biotinylated goat anti-mouse Vector Laboratories Cat No: BA-9200; RRID: AB_2336171 Chicken anti-Green Fluorescent Protein Aves Labs, Inc Cat No: GFP-1020; RRID: AB_10000240 Donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25810 Donkey anti-rabbit IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25701 Donkey anti-chicken Cy3 Jackson ImmunoResearch Labs Cat No: 703-165-155; RRID: AB_2340363 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Labs Cat No: 711-165-152; RRID: AB_2307443 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Labs Cat No: 711-225-152; RRID: AB_2340612 Donkey anti-sheep Cy5 Jackson ImmunoResearch Labs Cat No: 713-175-147; RRID: AB_2340730 Mouse anti-Tyrosine hydroxylase monoclonal Boehringer Mannheim Cat No: 1017-381 Rabbit anti-GABAAR a3 (extracellular) polyclonal Alomone Labs Cat No: AGA-003; RRID: AB_2039866 Rabbit anti-GABAAR a3 blocking peptide Alomone Labs Cat No: BLP-GA-003 Rabbit ant-GABAAR d (extracellular) Dr. W. Sieghart N/A Rabbit anti-Tyrosine hydroxylase polyclonal Millipore Cat No: AB152; RRID: AB_390204 Sheep anti-Tyrosine hydroxylase polyclonal Abcam Cat No: ab113; RRID: AB_297905 Bacterial and virus strains AAV8-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA pAAV-EF-1a-double floxedhChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth Addgene viral prep Cat No: 20297-AAV8 Chemicals, peptides, and recombinant proteins D-AP5 Hello Bio Cat No: HB0225 (+)Bicuculline Sigma-Aldrich Cat No: 14340 Calcium chloride dihydrate (CaCl2.2H2O) Sigma-Aldrich Cat No: C5080 DAB (3,30-Diaminobenzidine tetrahydrochloride) Sigma-Aldrich Cat No: D5905 DHbE hydrobromide Tocris Bioscience Cat No: 2349 DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat No: 276855 DNQX Hello Bio Cat No: HB0261 DOPAC (3,4-Dihydroxyphenylacetic acid) Sigma-Aldrich Cat No: D-9128 Dopamine hydrochloride (3- hydroxytryramine) Sigma-Aldrich Cat No: H-8502 EDTA (Ethylenediaminetetraacetic acid disodium salt dihydrate) Sigma-Aldrich Cat No: E1644 Ethanol, 200 proof Fisher Scientific Cat No: A962P-4 Euthasol Covetrus SKU No: 009444 Gabazine (SR 95531) Hello Bio Cat No: HB0901 D-(+)-Glucose Sigma-Aldrich Cat No: G-5767 HEPES (Acid) Sigma-Aldrich Cat No: H-3375 HEPES (Sodium Salt) Sigma-Aldrich Cat No: H-7006 Isoflurane Covetrus (NYULH-DCM) SKU No: 029405 (Continued on next page) 16 Cell Reports 43, 113834, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER JNJ-16259685 Tocris Bioscience Cat No: 2333 3-mercaptopropionic acid (3-MPA) Sigma-Aldrich Cat No: M5801 Magnesium sulfate heptahydrate (MgSO4.7H2O) Sigma-Aldrich Cat No: M-1880 Methanol Sigma-Aldrich Cat No: 34860 Muscimol Tocris Bioscience Cat No: 0289 Muscimol Hello Bio Cat No: HB0887 Normal Donkey Serum (NDS) Sigma-Aldrich Cat No: S30-M Paraformaldehyde Fisher Cat No: T353 Paraformaldehyde for EM (4% in 0.1M Phosphate Buffer, PH 7.4 Electron Microscopy Sciences Cat No: 15735-20S Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat No: P7059 Picrotoxin Sigma-Aldrich Cat No: P1675 Picrotoxin Hello Bio Cat No: HB0506 Potassium chloride (KCl) Sigma-Aldrich Cat No: P-3911 Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat No: P-0662 Sodium bicarbonate (NaHC03) Sigma-Aldrich Cat No: S-6014 Sodium-1-heptanesulfanate (C7H15O3SNa) Sigma-Aldrich Cat No: H2766 Sodium chloride (NaCl) Sigma-Aldrich Cat No: S-7653 Sodium octyl sulfate Sigma-Aldrich Cat No: O-4003 Sodium phosphate monobasic monohydrate (NaH2PO4.H2O) Sigma-Aldrich Cat No: S9638 Sucrose Sigma-Aldrich Cat No: S-7903 Strychnine hydrochloride Sigma-Aldrich Cat No: S8759 Triton X-100 Sigma-Aldrich Cat No: T8787 Critical commercial assays Silver Enhancer Kit for Microscopy Kirkegaard & Perry Laboratories (KPL), Inc Cat No: 50-22-01 Vectastain Elite ABC-HRP Kit Vector Labs Cat No: PK6100 Experimental models: Organisms/strains Mouse: Ai32 Jackson Laboratory Stock No: 024109 Mouse: C57BL/6J Jackson Laboratory Stock No: 000664 Mouse: DAT-IRES-Cre Jackson Laboratory Stock No: 006660 Mouse: GAT1flox/flox Tritsch Lab, NYULH geneOway JAX stock No: 037219 Software and algorithms DropViz RNA-Seq Data Saunders et al.48 https://DropViz.org Fiji ImageJ NIH https://imagej.nih.gov/ij/ Fusion Benchtop Image Acquisition Software (ver. .. 1.101) Andor Technology Ltd. Oxford Instruments https://andor.oxinst.com/ Imaris Microscope Imaging Software (ver.

    Microscopy:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated donkey anti-rabbit Jackson ImmunoResearch Labs Cat No: 711-065-152; RRID: AB_2340593 Biotinylated goat anti-mouse Vector Laboratories Cat No: BA-9200; RRID: AB_2336171 Chicken anti-Green Fluorescent Protein Aves Labs, Inc Cat No: GFP-1020; RRID: AB_10000240 Donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25810 Donkey anti-rabbit IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25701 Donkey anti-chicken Cy3 Jackson ImmunoResearch Labs Cat No: 703-165-155; RRID: AB_2340363 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Labs Cat No: 711-165-152; RRID: AB_2307443 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Labs Cat No: 711-225-152; RRID: AB_2340612 Donkey anti-sheep Cy5 Jackson ImmunoResearch Labs Cat No: 713-175-147; RRID: AB_2340730 Mouse anti-Tyrosine hydroxylase monoclonal Boehringer Mannheim Cat No: 1017-381 Rabbit anti-GABAAR a3 (extracellular) polyclonal Alomone Labs Cat No: AGA-003; RRID: AB_2039866 Rabbit anti-GABAAR a3 blocking peptide Alomone Labs Cat No: BLP-GA-003 Rabbit ant-GABAAR d (extracellular) Dr. W. Sieghart N/A Rabbit anti-Tyrosine hydroxylase polyclonal Millipore Cat No: AB152; RRID: AB_390204 Sheep anti-Tyrosine hydroxylase polyclonal Abcam Cat No: ab113; RRID: AB_297905 Bacterial and virus strains AAV8-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA pAAV-EF-1a-double floxedhChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth Addgene viral prep Cat No: 20297-AAV8 Chemicals, peptides, and recombinant proteins D-AP5 Hello Bio Cat No: HB0225 (+)Bicuculline Sigma-Aldrich Cat No: 14340 Calcium chloride dihydrate (CaCl2.2H2O) Sigma-Aldrich Cat No: C5080 DAB (3,30-Diaminobenzidine tetrahydrochloride) Sigma-Aldrich Cat No: D5905 DHbE hydrobromide Tocris Bioscience Cat No: 2349 DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat No: 276855 DNQX Hello Bio Cat No: HB0261 DOPAC (3,4-Dihydroxyphenylacetic acid) Sigma-Aldrich Cat No: D-9128 Dopamine hydrochloride (3- hydroxytryramine) Sigma-Aldrich Cat No: H-8502 EDTA (Ethylenediaminetetraacetic acid disodium salt dihydrate) Sigma-Aldrich Cat No: E1644 Ethanol, 200 proof Fisher Scientific Cat No: A962P-4 Euthasol Covetrus SKU No: 009444 Gabazine (SR 95531) Hello Bio Cat No: HB0901 D-(+)-Glucose Sigma-Aldrich Cat No: G-5767 HEPES (Acid) Sigma-Aldrich Cat No: H-3375 HEPES (Sodium Salt) Sigma-Aldrich Cat No: H-7006 Isoflurane Covetrus (NYULH-DCM) SKU No: 029405 (Continued on next page) 16 Cell Reports 43, 113834, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER JNJ-16259685 Tocris Bioscience Cat No: 2333 3-mercaptopropionic acid (3-MPA) Sigma-Aldrich Cat No: M5801 Magnesium sulfate heptahydrate (MgSO4.7H2O) Sigma-Aldrich Cat No: M-1880 Methanol Sigma-Aldrich Cat No: 34860 Muscimol Tocris Bioscience Cat No: 0289 Muscimol Hello Bio Cat No: HB0887 Normal Donkey Serum (NDS) Sigma-Aldrich Cat No: S30-M Paraformaldehyde Fisher Cat No: T353 Paraformaldehyde for EM (4% in 0.1M Phosphate Buffer, PH 7.4 Electron Microscopy Sciences Cat No: 15735-20S Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat No: P7059 Picrotoxin Sigma-Aldrich Cat No: P1675 Picrotoxin Hello Bio Cat No: HB0506 Potassium chloride (KCl) Sigma-Aldrich Cat No: P-3911 Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat No: P-0662 Sodium bicarbonate (NaHC03) Sigma-Aldrich Cat No: S-6014 Sodium-1-heptanesulfanate (C7H15O3SNa) Sigma-Aldrich Cat No: H2766 Sodium chloride (NaCl) Sigma-Aldrich Cat No: S-7653 Sodium octyl sulfate Sigma-Aldrich Cat No: O-4003 Sodium phosphate monobasic monohydrate (NaH2PO4.H2O) Sigma-Aldrich Cat No: S9638 Sucrose Sigma-Aldrich Cat No: S-7903 Strychnine hydrochloride Sigma-Aldrich Cat No: S8759 Triton X-100 Sigma-Aldrich Cat No: T8787 Critical commercial assays Silver Enhancer Kit for Microscopy Kirkegaard & Perry Laboratories (KPL), Inc Cat No: 50-22-01 Vectastain Elite ABC-HRP Kit Vector Labs Cat No: PK6100 Experimental models: Organisms/strains Mouse: Ai32 Jackson Laboratory Stock No: 024109 Mouse: C57BL/6J Jackson Laboratory Stock No: 000664 Mouse: DAT-IRES-Cre Jackson Laboratory Stock No: 006660 Mouse: GAT1flox/flox Tritsch Lab, NYULH geneOway JAX stock No: 037219 Software and algorithms DropViz RNA-Seq Data Saunders et al.48 https://DropViz.org Fiji ImageJ NIH https://imagej.nih.gov/ij/ Fusion Benchtop Image Acquisition Software (ver. .. 1.101) Andor Technology Ltd. Oxford Instruments https://andor.oxinst.com/ Imaris Microscope Imaging Software (ver.

    Software:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated donkey anti-rabbit Jackson ImmunoResearch Labs Cat No: 711-065-152; RRID: AB_2340593 Biotinylated goat anti-mouse Vector Laboratories Cat No: BA-9200; RRID: AB_2336171 Chicken anti-Green Fluorescent Protein Aves Labs, Inc Cat No: GFP-1020; RRID: AB_10000240 Donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25810 Donkey anti-rabbit IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25701 Donkey anti-chicken Cy3 Jackson ImmunoResearch Labs Cat No: 703-165-155; RRID: AB_2340363 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Labs Cat No: 711-165-152; RRID: AB_2307443 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Labs Cat No: 711-225-152; RRID: AB_2340612 Donkey anti-sheep Cy5 Jackson ImmunoResearch Labs Cat No: 713-175-147; RRID: AB_2340730 Mouse anti-Tyrosine hydroxylase monoclonal Boehringer Mannheim Cat No: 1017-381 Rabbit anti-GABAAR a3 (extracellular) polyclonal Alomone Labs Cat No: AGA-003; RRID: AB_2039866 Rabbit anti-GABAAR a3 blocking peptide Alomone Labs Cat No: BLP-GA-003 Rabbit ant-GABAAR d (extracellular) Dr. W. Sieghart N/A Rabbit anti-Tyrosine hydroxylase polyclonal Millipore Cat No: AB152; RRID: AB_390204 Sheep anti-Tyrosine hydroxylase polyclonal Abcam Cat No: ab113; RRID: AB_297905 Bacterial and virus strains AAV8-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA pAAV-EF-1a-double floxedhChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth Addgene viral prep Cat No: 20297-AAV8 Chemicals, peptides, and recombinant proteins D-AP5 Hello Bio Cat No: HB0225 (+)Bicuculline Sigma-Aldrich Cat No: 14340 Calcium chloride dihydrate (CaCl2.2H2O) Sigma-Aldrich Cat No: C5080 DAB (3,30-Diaminobenzidine tetrahydrochloride) Sigma-Aldrich Cat No: D5905 DHbE hydrobromide Tocris Bioscience Cat No: 2349 DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat No: 276855 DNQX Hello Bio Cat No: HB0261 DOPAC (3,4-Dihydroxyphenylacetic acid) Sigma-Aldrich Cat No: D-9128 Dopamine hydrochloride (3- hydroxytryramine) Sigma-Aldrich Cat No: H-8502 EDTA (Ethylenediaminetetraacetic acid disodium salt dihydrate) Sigma-Aldrich Cat No: E1644 Ethanol, 200 proof Fisher Scientific Cat No: A962P-4 Euthasol Covetrus SKU No: 009444 Gabazine (SR 95531) Hello Bio Cat No: HB0901 D-(+)-Glucose Sigma-Aldrich Cat No: G-5767 HEPES (Acid) Sigma-Aldrich Cat No: H-3375 HEPES (Sodium Salt) Sigma-Aldrich Cat No: H-7006 Isoflurane Covetrus (NYULH-DCM) SKU No: 029405 (Continued on next page) 16 Cell Reports 43, 113834, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER JNJ-16259685 Tocris Bioscience Cat No: 2333 3-mercaptopropionic acid (3-MPA) Sigma-Aldrich Cat No: M5801 Magnesium sulfate heptahydrate (MgSO4.7H2O) Sigma-Aldrich Cat No: M-1880 Methanol Sigma-Aldrich Cat No: 34860 Muscimol Tocris Bioscience Cat No: 0289 Muscimol Hello Bio Cat No: HB0887 Normal Donkey Serum (NDS) Sigma-Aldrich Cat No: S30-M Paraformaldehyde Fisher Cat No: T353 Paraformaldehyde for EM (4% in 0.1M Phosphate Buffer, PH 7.4 Electron Microscopy Sciences Cat No: 15735-20S Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat No: P7059 Picrotoxin Sigma-Aldrich Cat No: P1675 Picrotoxin Hello Bio Cat No: HB0506 Potassium chloride (KCl) Sigma-Aldrich Cat No: P-3911 Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat No: P-0662 Sodium bicarbonate (NaHC03) Sigma-Aldrich Cat No: S-6014 Sodium-1-heptanesulfanate (C7H15O3SNa) Sigma-Aldrich Cat No: H2766 Sodium chloride (NaCl) Sigma-Aldrich Cat No: S-7653 Sodium octyl sulfate Sigma-Aldrich Cat No: O-4003 Sodium phosphate monobasic monohydrate (NaH2PO4.H2O) Sigma-Aldrich Cat No: S9638 Sucrose Sigma-Aldrich Cat No: S-7903 Strychnine hydrochloride Sigma-Aldrich Cat No: S8759 Triton X-100 Sigma-Aldrich Cat No: T8787 Critical commercial assays Silver Enhancer Kit for Microscopy Kirkegaard & Perry Laboratories (KPL), Inc Cat No: 50-22-01 Vectastain Elite ABC-HRP Kit Vector Labs Cat No: PK6100 Experimental models: Organisms/strains Mouse: Ai32 Jackson Laboratory Stock No: 024109 Mouse: C57BL/6J Jackson Laboratory Stock No: 000664 Mouse: DAT-IRES-Cre Jackson Laboratory Stock No: 006660 Mouse: GAT1flox/flox Tritsch Lab, NYULH geneOway JAX stock No: 037219 Software and algorithms DropViz RNA-Seq Data Saunders et al.48 https://DropViz.org Fiji ImageJ NIH https://imagej.nih.gov/ij/ Fusion Benchtop Image Acquisition Software (ver. .. 1.101) Andor Technology Ltd. Oxford Instruments https://andor.oxinst.com/ Imaris Microscope Imaging Software (ver.

    RNA sequencing:

    Article Title: GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA A receptors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated donkey anti-rabbit Jackson ImmunoResearch Labs Cat No: 711-065-152; RRID: AB_2340593 Biotinylated goat anti-mouse Vector Laboratories Cat No: BA-9200; RRID: AB_2336171 Chicken anti-Green Fluorescent Protein Aves Labs, Inc Cat No: GFP-1020; RRID: AB_10000240 Donkey anti-mouse IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25810 Donkey anti-rabbit IgG conjugated to ultrasmall (0.8 nm) colloidal gold Electron Microscopy Sciences Cat No: 25701 Donkey anti-chicken Cy3 Jackson ImmunoResearch Labs Cat No: 703-165-155; RRID: AB_2340363 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Labs Cat No: 711-165-152; RRID: AB_2307443 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Labs Cat No: 711-225-152; RRID: AB_2340612 Donkey anti-sheep Cy5 Jackson ImmunoResearch Labs Cat No: 713-175-147; RRID: AB_2340730 Mouse anti-Tyrosine hydroxylase monoclonal Boehringer Mannheim Cat No: 1017-381 Rabbit anti-GABAAR a3 (extracellular) polyclonal Alomone Labs Cat No: AGA-003; RRID: AB_2039866 Rabbit anti-GABAAR a3 blocking peptide Alomone Labs Cat No: BLP-GA-003 Rabbit ant-GABAAR d (extracellular) Dr. W. Sieghart N/A Rabbit anti-Tyrosine hydroxylase polyclonal Millipore Cat No: AB152; RRID: AB_390204 Sheep anti-Tyrosine hydroxylase polyclonal Abcam Cat No: ab113; RRID: AB_297905 Bacterial and virus strains AAV8-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA pAAV-EF-1a-double floxedhChR2(H134R)-mCherry-WPRE-HGHpA was a gift from Karl Deisseroth Addgene viral prep Cat No: 20297-AAV8 Chemicals, peptides, and recombinant proteins D-AP5 Hello Bio Cat No: HB0225 (+)Bicuculline Sigma-Aldrich Cat No: 14340 Calcium chloride dihydrate (CaCl2.2H2O) Sigma-Aldrich Cat No: C5080 DAB (3,30-Diaminobenzidine tetrahydrochloride) Sigma-Aldrich Cat No: D5905 DHbE hydrobromide Tocris Bioscience Cat No: 2349 DMSO (dimethyl sulfoxide) Sigma-Aldrich Cat No: 276855 DNQX Hello Bio Cat No: HB0261 DOPAC (3,4-Dihydroxyphenylacetic acid) Sigma-Aldrich Cat No: D-9128 Dopamine hydrochloride (3- hydroxytryramine) Sigma-Aldrich Cat No: H-8502 EDTA (Ethylenediaminetetraacetic acid disodium salt dihydrate) Sigma-Aldrich Cat No: E1644 Ethanol, 200 proof Fisher Scientific Cat No: A962P-4 Euthasol Covetrus SKU No: 009444 Gabazine (SR 95531) Hello Bio Cat No: HB0901 D-(+)-Glucose Sigma-Aldrich Cat No: G-5767 HEPES (Acid) Sigma-Aldrich Cat No: H-3375 HEPES (Sodium Salt) Sigma-Aldrich Cat No: H-7006 Isoflurane Covetrus (NYULH-DCM) SKU No: 029405 (Continued on next page) 16 Cell Reports 43, 113834, March 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER JNJ-16259685 Tocris Bioscience Cat No: 2333 3-mercaptopropionic acid (3-MPA) Sigma-Aldrich Cat No: M5801 Magnesium sulfate heptahydrate (MgSO4.7H2O) Sigma-Aldrich Cat No: M-1880 Methanol Sigma-Aldrich Cat No: 34860 Muscimol Tocris Bioscience Cat No: 0289 Muscimol Hello Bio Cat No: HB0887 Normal Donkey Serum (NDS) Sigma-Aldrich Cat No: S30-M Paraformaldehyde Fisher Cat No: T353 Paraformaldehyde for EM (4% in 0.1M Phosphate Buffer, PH 7.4 Electron Microscopy Sciences Cat No: 15735-20S Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat No: P7059 Picrotoxin Sigma-Aldrich Cat No: P1675 Picrotoxin Hello Bio Cat No: HB0506 Potassium chloride (KCl) Sigma-Aldrich Cat No: P-3911 Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat No: P-0662 Sodium bicarbonate (NaHC03) Sigma-Aldrich Cat No: S-6014 Sodium-1-heptanesulfanate (C7H15O3SNa) Sigma-Aldrich Cat No: H2766 Sodium chloride (NaCl) Sigma-Aldrich Cat No: S-7653 Sodium octyl sulfate Sigma-Aldrich Cat No: O-4003 Sodium phosphate monobasic monohydrate (NaH2PO4.H2O) Sigma-Aldrich Cat No: S9638 Sucrose Sigma-Aldrich Cat No: S-7903 Strychnine hydrochloride Sigma-Aldrich Cat No: S8759 Triton X-100 Sigma-Aldrich Cat No: T8787 Critical commercial assays Silver Enhancer Kit for Microscopy Kirkegaard & Perry Laboratories (KPL), Inc Cat No: 50-22-01 Vectastain Elite ABC-HRP Kit Vector Labs Cat No: PK6100 Experimental models: Organisms/strains Mouse: Ai32 Jackson Laboratory Stock No: 024109 Mouse: C57BL/6J Jackson Laboratory Stock No: 000664 Mouse: DAT-IRES-Cre Jackson Laboratory Stock No: 006660 Mouse: GAT1flox/flox Tritsch Lab, NYULH geneOway JAX stock No: 037219 Software and algorithms DropViz RNA-Seq Data Saunders et al.48 https://DropViz.org Fiji ImageJ NIH https://imagej.nih.gov/ij/ Fusion Benchtop Image Acquisition Software (ver. .. 1.101) Andor Technology Ltd. Oxford Instruments https://andor.oxinst.com/ Imaris Microscope Imaging Software (ver.



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